The Role of Neuroimmunoregulation Mobilized by Enhancing Immunologic Function Via Hou Hai Acupoint Antigen Injection
- VernacularTitle:抗原穴位注射增强机体免疫功能的神经免疫调节作用
- Author:
Yang GAO
1
;
Jian-Zhong WANG
;
Yee-Kong NG
;
Jia-You LIN
;
Li LI
;
Dong WANG
;
Eng-Ang LIN
Author Information
1. 中国医学科学院/中国协和医科大学基础医学研究所
- From:
Acta Academiae Medicinae Sinicae
2001;23(2):163-167
- CountryChina
- Language:Chinese
-
Abstract:
Department of Immunology; Institute of Basic Medical Science, CAMS and PUMC, Beijing 100005, China) Objective To study the mechanism of neuroimmunoregulation which is triggered by enhancing immunologic function via Hou Hai acupoint antigen injection. Methods Immunohistochemical method, immunofluorometric method and RT-PCR were used to examine the different distribution of cy tokine immunopositive cells in the brain and expression of cytokines in the spleen of the human IgG sensitized rats recieved acupoint, subcutaneous and normal rats antigen injections. Results In the areas of lateral hypothalamic nucleus (LH) and amygdaloid nuclear complex(AA) , the distribution of cytokines immunopositive cells with acupoint injection group was significantly increased more than that in the subcutaneous injection group. But the expression of cytokines immunopositive cells both by the acu point injection and the subcutaneous injection groups reached their peak value in similar time. Dou ble-labelling results showed that the cytokine immunopositive cells were neurons. In the spleens, the expressions of cytokines, IL-2 and IFN, were significantly increased by acupoint injection more than subcutaneous injection. Conclusions The time course of neuroimmunoregulation is similar in the im munized rats via both acupoint injection and subcutaneous injection of antigens. But the activity of neuroimmunoregulation is not the same in acupoint and subcutaneous injection groups. Neurons of the LH and AA are main source spf teh neruroimunomodulation The effect of enhancing immunlolgic funivtion via Hou acupoin injection is may be more efficent to mobnilize the activity of neitoimmunreguation of immune-asociated brain than moduation the immune system
