Eukaryotic Expression and in vitro Tumor Cell Apoptosis-induced Effect of Human TRAIL
- VernacularTitle:人TRAIL cDNA的真核表达和体外肿瘤细胞的凋亡诱导作用
- Author:
Xu SHI
1
;
Jia-Qiang HUANG
;
Ying-Mei ZHANG
;
Quan-Sheng SONG
;
Da-Long MA
Author Information
1. 北京大学
- From:
Acta Academiae Medicinae Sinicae
2001;23(2):145-149
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the tumor cell apoptosis induced by the effect in vitro of eukaryotic ex pression of full-length TRAIL cDNA and its extracellular region. Methods The eukaryotic expression vectors for both forms of the cDNA acquired from the fetal heart cDNA library were constructed. After gene transfecting, the stable expression cell lines were obtained by G418 screening. Results The supernatants from tansfectants could induce apoptosis of different tumor cell lines in vitro, and an enhanced effect was observed by adding TFAR19 (TF-1 cell apoptosis-related protein 19), a novel apoptosis gene product discovered in our laboratory. Conclusion Eukaryotic expression products of TRAIL can induce apoptosis of the tumor cells, and TFAR19 could enhance the effect on apoptosis of tumor cells.
