Expression of Neurotrophin-4 (NT-4) Gene in Baculovirus Expression Vector System
- VernacularTitle:神经营养素-4(NT-4)在昆虫杆状病毒表达系统中的克隆与表达
- Author:
Xiao-Li MA
1
;
Bing-Ren HUANG
Author Information
1. CAMS and PUMC
- From:
Acta Academiae Medicinae Sinicae
2001;23(2):141-144
- CountryChina
- Language:Chinese
-
Abstract:
Objective We selected baculovirus expression vector system to express human NT-4 with biological activity. Methods The hNT-4 mature peptide-coding sequence is amplified by polymerase chain reaction (PCR), ligated to baculovirus expression vector PacGP67B, and expressed in the insert sf9 cell line. Results The protein molecular weight of the expressed hNT-4 was about 15000 by SDS-PAGE,and the hNT-4 antibody could react with this protein in the infected supernatant and total cell by western-blot . The activity of hNT-4 determined by PC12 cell line was definite. Conclusions The results may aid for studying the effect of the hNT-4 on basic medical research and clinical application.
