Expression in E.coli and Chemotactic Activity Analyzing of Recombinant CC-chemokine RANTES
- VernacularTitle:重组CC家族趋化因子RANTES的原核表达及趋化活性分析
- Author:
Rong-Hua SUN
1
;
Ying WANG
;
Ying-Cheng ZHONG
;
Da-Long MA
Author Information
1. 北京大学
- From:
Acta Academiae Medicinae Sinicae
2001;23(2):123-126
- CountryChina
- Language:Chinese
-
Abstract:
Objective To further understand the structure and function of RANTES and provide basis for research for application. Methods PCR product corresponding to region encoding mature RANTES protein was cloned into E.coli expression vector;, purified recombinant protein was obtained by heparin affinity chro matography and its chemotactic activity was determined by Boyden chamber. Results The recombinant RANTES was expressed in E.coli with high efficiency. Purified protein showed chemotactic activity to pe ripheral blood lymphocytes, U937 cells, and CCR4 stable-transfected HEK293 cells. Conclusion The puri fled recombinant protein exerted chemotactic activity on PBL and cultured cell lines, and we have estab lished a experimental system for further study of structure and function of RANTES.
