Stimulatory Effect of Chemokine-like Factor 1 (CKLF1) on the Growth of Bone Marrow Cells
- VernacularTitle:趋化素样因子(CKLF1)对骨髓细胞增殖活性的研究
- Author:
Wen-Ling HAN
1
;
Min RUI
;
Ying-Mei ZHANG
;
Ying-Yu CHEN
;
Ying-Cheng ZHONG
;
Chun-Hui DI
;
Quan-Sheng SONG
;
Da-Long MA
Author Information
1. 北京大学
- From:
Acta Academiae Medicinae Sinicae
2001;23(2):119-122
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the activity of CKLF1 on the proliferation and differentiation of bone marrow cells. Methods Human low density bone marrow cells and mouse bone marrow cells were plated in 96-well microplate and supernatants from transfected COS-7 cell culture were added. The cell proliferation was assayed by MTT method after 5 days incubation. The enhancing effect of CKLF1 on the colony forma tion of human hematopoietic progenitor cells was identified in semi-solid culture. Results CKLF1 has obvi ous enhancing effect on both human and mouse bone marrow cells, it can stimulate the colony formation of human hematopoietic stem cells and has synergistic action with GM-CSF. Conclusion CKLF1 can pro mote the proliferation and differentiation of bone marrow cells.
