Roles of G-Protein β and γ Subunits.in the Interaction of Gβγ with Adenylyl Cyclases Ⅱ
- VernacularTitle:Gβ，Gβγ与腺苷酸环化酶Ⅱ在酵母双杂交和三杂交系统中的相互作用
- Author:
Jing-Jun LI
1
;
Ning LU
;
Bing-Ren HUANG
Author Information
1. CAMS and PUMC
- From:
Acta Academiae Medicinae Sinicae
2001;23(2):115-118
- CountryChina
- Language:Chinese
-
Abstract:
To explore the individual roles of G protein β and γsubunit in the interactions with effectors. Methods We investigated the interactions of Gβ1 and Gβ1γ2 with adenylyl cyclase Ⅱ(ACⅡ) us ing the yeast two-hybrid and three-hybrid systems. Results When assayed for the abilities to activate the reporter gene, the interactions among AD-β1, γ2 and BD-AC ⅡQ in the three-hybrid system were more po tent than the interactions between AD-β1 and BD-AC ⅡQ in the two-hybrid system. The expressions of BD-AC ⅡQ and AD-β1 in transformants coexpressed AD-β1 and BD-AC ⅡQ, and transformants coexpressed AD-β1, γ2 and BD-AC ⅡQ were respectively detected. The comparisons between the reporter activity and the expression levels of BD-AC ⅡQ and AD-β1 in the yeast cells show there was no correlations, i.e.The difference in the reporter activity was not a reflection of differential expression level of the hybrid pro teins. Conclutions All these results suggest that G protein β1 subunit is sufficient to maintain the basic in teraction between Gβ1γ2 and AC ⅡQ, and γ2 subunit plays an important role in the high affinity interaction of Gβ1γ2 with ACⅡQ.
