Hunting for Novel Protein Factors in G-protein Pathway with Yeast Two-hybrid System
- VernacularTitle:应用酵母双杂交研究G蛋白通路中的蛋白因子
- Author:
Li Ning LU
1
;
Jing-jun
;
Bing-Ren HUANG
Author Information
1. Institute of Basic Medical Sciences
- From:
Acta Academiae Medicinae Sinicae
2001;23(2):111-1114
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the protein factors that could interact with Gβ subunit within the G protein sig nal transducing pathway. Methods The highly sensitive protein-protein interaction system——Yeast Two-hybrid System was applied to screen the human cDNA library with constructed “Bite plasmid” contain ing Gβ subunit gene fragment. And then the faulse positive test was adapted. Results Three positive gene fragments were obtained. One codes for “Actin bundling protein”. The other two are new ones and their GeneBank accession numbers are AF288405 and AF288406 respectively. Conclusions It is the first time to find that among human brain tissue, Gβ subunit becomes a structural or functional unit interacts with actin bundling protein and the other two unknown protein factors to activate the following pathway. This re sult may be important to understand the relationship between the actin cytoskeleton and G proteins.
