Fudenine, Relative to Blood Glucose-control, Is a Novel Membrane Protein
- VernacularTitle:Fudenine——一个新的与血糖调节相关的膜蛋白
- Author:
Yong-Sheng CHANG
1
;
JIN ZUO
;
Xue-Feng ZHANG
;
Ming ZHANG
;
Fu-De FANG
Author Information
1. CAMS and PUMC
- From:
Acta Academiae Medicinae Sinicae
2001;23(1):63-64
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine whether Fudenine is a novel membrane protein. Methods Green fluorescence protein(GFP)was used to localize Fudenine in vivo. GFP, as a control, was targeted to cytoplasm.Epithelial cell, CBRH7919, and non-epithelial cell, L-6TG, were cultured and transiently transfected by using the lipofectamine reagent. After 48 h, intact cells were examined with fluorescence microscope for Fudenine. Results Reporter plasmid pEGFP-N1, as a control , was expressed and localized to cytoplasm. But Fudenine, driven by the cytomegalovirus promoterenhancer contained in the pEGFP-N1 vector, was overexpressed and targeted to cellular membrane. Conclusions Fudenine is a novel membrane protein. It may play the similar role with its homologues AC133 antigen and prominin in human and mouse.respectively. It might be involved in signaling transduction and regulate blood glucose metabolism in vivo.
