Inhibitory Effect of Melatonin on the Development of Pituitary Prolactin-producing Tumors Induced by 17-β-Estradiol
- VernacularTitle:褪黑素抑制由17-β-雌二醇诱发的垂体催乳素瘤的生长
- Author:
Lie GAO
1
;
Rong-Kun XU
;
C.S.Pang
;
Jian-Ping XU
;
Hui-Min SHAN
;
S.F.Pang
Author Information
1. Institute of Basic Medical Sciences
- From:
Acta Academiae Medicinae Sinicae
2001;23(1):49-53
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine the inhibitory effect of melatonin (MLT) on the development of pituitary prolactin-producing tumors (prolactinoma) induced by 17-β-estradiol (E2), in vivo, and explore MLT′s oncostatic mechanisms. Methods The prolactinomas were established by implanting E2-1aden silastic capsules subcutaneously in Sprague-Dawley male rats. MLT doses 0.05, 0.25, 0.50, 1.00, and 2.00 mg/rat were administrated separately to 5 groups subcutaneously starting seven days prior to tumor induction for 97 days. The matched controls were given equal volumes of 4% alcohol in saline. Results (1) The prolactinoma weights in 0.05, 0.25, 0.50, 1.00 and 2.00 mg MLT dose groups were 25.91% (P＞0.05), 48.78% (P ＜0. 01), 36.78% (P ＜0.05), 31.04% (P＞ 0.05) and35.22% (P＞ 0.05)respectively which were lower than that of control group;(2) The PRL mRNA levels of prolactinoma in 0.05,0.25,and 0.50mg MLT dose groups were 33.67% (P＜0.05), 25.51% (P＜0.05) and 41.84%(P＜0.01)respectively which were lower than that of control group as estimated by Northern Blot, and the in situ hybridization studies; (3) The DNA contents of prolactinoma in 0. 05, 0. 25 and 0. 50 mg MLT dose groups were 40. 73% (P＜0.001), 51.15% (P＜0.001) and 60. 23% (P ＜0. 001) respectively which were lower than that of control group by laser scanning confocal microscopy; (4) Plasma peroxidative lipid contents in 0.05,0.25,0.50,1.00 and 2.00 mg MLT dose groups were 26.45% (P ＜0.05), 23.97% (P ＜0.05), 47. 11%(P ＜0.001), 66. 12%(P ＜0.001)and64.46% (P ＜0.001)respectively which were lower than that of control group. The correlation coefficient between MLT doses and plasma peroxidative lipid contents was - 0.8257 (P＜0.05). Conclusions MLT in suitable doses is able to inhibit the development of E2-induced prolactinoma by inhibiting the expression of PRL gene and the DNA synthesis. The link between MLT antioxidative action and its inhibitory effect on development of prolactinoma should be further investigated.
