Polymorphism of Parathyroid Hormone Gene And Its Association with Bone Mineral Density of Han Nationality Women in Beijing Area
- VernacularTitle:北京地区汉族成人甲状旁腺素基因多态性与骨矿盐密度的相关性
- Author:
Mei LI
1
;
Xun-Wu MENG
;
Xue-Ying ZHOU
;
Xiao-Ping XING
Author Information
1. PUMC Hospital CAMS and PUMC
- From:
Acta Academiae Medicinae Sinicae
2001;23(1):45-48
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the distribution of frequency of parathyroid hormone (PTH) gene polymorphisms in healthy adults of Han nationality in Bejing area and relationship between PTH genotypes and bone mineral density(BMD) in young and postmenopausal women. Methods Polymorphisms of PTH gene were detected by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) of restriction enzyme Bst B1 in 270 subjects. If the site of enzyme Bst B1 existed, the PTH genotype was “B”. On the contrary, if base mutation occurred, the genotype was “b”. Some of the PTH genotypes were confirmed by DNA sequences analysis. Bone mineral density (BMD) was measured by dual-energy X-ray absorptiome try. Results Genotype frequencies of BB, Bb, bb were 73.7% , 25.9% , and 0. 4% respectively in adults of Beijing areas. The frequencies of RFLP alleles B, b were 86. 7% and 13.3%. Beijing postmenopausal women frequencies of BB, Bb, bb were 67.1%, 32.2% and 0.7%. B, b alleles frequencies were 83.2% and 16. 8%. We statistically compared bone mineral density at the lumbar 2-4, neck,wards triangle and trochanter major, there was no significant difference between BB and Bb genotype of young women and postmenopausal women groups. Otherwise, no obvious relationship was found between the BMD and PTH genotype in Beijing women. Conclusions PTH gene polymorphisms were not associateds with BMD in Beijing women.
