Development of a Canine Adenovirus Type 1 Vaccine Strain E3-deleted Based Expression Vector
- VernacularTitle:犬腺病毒1型疫苗株E3缺失表达载体的构建
- Author:
Hao LI
1
;
Qi-Yi TANG
;
Yun ZHANG
;
Wang YUN
;
Cai-Yun GUO
Author Information
1. Institute of Basic Medical Sciences, CAMS and PUMC
- From:
Acta Academiae Medicinae Sinicae
2001;23(1):40-44
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate canine adenovirus type 1 vaccine strain (Cannaught Laboratory Limited,CLL) as recombinant vaccine and gene transfer vector. Methods Recombinant virus CLLEGFP which contains enhanced green fluorescent protein(EGFP) reporter gene was constructed. CLLEGFP was used to infect various human derived cell lines (293, Hela, CO, SW, Hep-2 and CAM) by inoculating intraperitoneally(IP), intravenously(IV)and intramuscularly (IM)to Kunming mice other than oral administration. Various tissue samples of the mice were collected at multitime point for observing EGFP green fluorescence. Anti-EGFP antibodies were detected by Western blot analysis in the sera after 4 weeks. Results CLLEGFP can infect various human derived cell lines and express EGFP. EGFP green fluorescence were observed in liver tissue cells after IP transducing 3 days. All immune inoculation ways above could induce Kunming mice producing anti-EGFP antibodies which were identified by Western blot analysis. Conclusions These resluts indicate that CLL possess powerful potential as recombinant vaccine and gene transfer vector.
