Gene Cloning and Expression of Human Soluble Interleukin-6 Receptor in Inssect Cells
- VernacularTitle:人可溶性白细胞介素-6受体基因在昆虫细胞内的克隆与表达
- Author:
Bing-Sheng CHANG
1
;
Yuan LI
Author Information
1. Institute of Medicinal Biotechnology
- From:
Acta Academiae Medicinae Sinicae
2001;23(1):36-39
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clone the human soluble interleukin-6 receptor(hsIL-6R) gene and expression in insect cell line. Methods The hsIL-6R gene was cloned into plasmid pAcGP67B. After co-transfection of recombinant plasmid and wild type AcNPV DNA, the rAcNPV was confirmed by the end point dilution assay and dot blot. Then it was purified by plaque assay. Results SDS-PAGE showed molecular weight of the expressed product was about 47 000. The expressed recombinant protein was confirmed to be specific and capable of binding its ligand IL-6 by Western blot and ligand-receptor binding assays. Conclusions Secretory expression of hsIL-6R gene in baculovirus expression system was indicated. The expressed product had immunological and biological activities.
