Differentiation and Malignant Suppression Induced by Mouse Erythroid Differentiation and Denucleation Factor on Mouse Erythroleukemia Cells
- VernacularTitle:红细胞分化去核因子在诱导小鼠红白血病细胞分化以及恶性逆转中的作用
- Author:
Qing ZHAO
1
;
Ye-Hua GE
;
Jian-Ping ZHOU
;
Jing MA
;
Ke-Quan CHEN
;
She-Pu XUE
;
Dai-Shu HAN
Author Information
1. Institute of Basic Medical Sciences
- From:
Acta Academiae Medicinae Sinicae
2001;23(1):32-35
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the roles of mouse erythroid differentiation and denucleation factor (MEDDF), newly cloned in our laboratory, in erythroid terminal differentiation. Methods Mouse erythroleukemia cells (MEL) were transfected with eukaryotic expression plasmid pcDNA-MEDDF. The changes of cell growth rate, mitotic index and colony-forming rate in semi-solid medium were investigated.The expressions of c-myc and β-globin genes were analysed by semi-quantitative RT-PCR. Results MEL cells transfected with pcDNA-MEDDF showed significant lower growth rate, mitotic index, and colony-forming rate in semisolid medium(P＜0.01).The percentage of benzidine-positive cells was 32.8% after transfection. The expression of β-globin in cells transfected with pcDNA-MEDDF was 3.43 times higher than that of control (MEL transfected with blank vector, pcDNA3.1 ), and the expression of c-myc was decreased by 66.3%.Conclusions MEDDF can induce differentiation of MELcell, and suppress its malignancy likely.
