Cloning of Human B Lymphocyte Activation-related Novel Gene
- VernacularTitle:人B淋巴细胞活化相关新基因的克隆
- Author:
Xing-Wu LU
1
;
Ji-Yi YIN
;
Lian-Xian CUI
Author Information
1. CAMS and PUMC
- From:
Acta Academiae Medicinae Sinicae
2001;23(1):27-31
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clone the novel activation-related gene of B lymphocyte. Methods The differential display reversal transcription PCR (DDRT-PCR) technique was applied to analyse the expression difference of mRNA between resting and activated B lymphocyte from human tonsil. The positive differential display cDNA fragment identified by Northern-blotting was chosen as probe to filtrate human activated B lymphocyte cDNA library. Results Sixty two differential display cDNA fragments(expressed sequence tag, EST)were obtained. Thirty-two of them were mainly expressed in resting B lymphocyts and thirty were expressed in activated cells. Twenty-five were positive ones after identification by Northern blot analysis. A novel cDNA clone was obtained after using EST30 as a probe to filtrate the human activated B cell cDNA library.The whole cDNA clone was 2 048 bp in length and contains a 630 bp open reading frame. The N end of the deduced amino acid sequence was homologous with KAR3 protein which is a member of kinesins superfamily in yeast. Conclusions A novel possible activation-related gene in human B lymphocyte was obtained.
