Fibrinolytic effect of curdione in dissolving deep vein thrombosis in rats
10.12173/j.issn.2097-4922.202406061
- VernacularTitle:莪术二酮溶解大鼠深静脉血栓的纤溶作用
- Author:
Hanbing SHI
1
;
Teng ZHANG
;
Shan DU
;
Yuxuan GUAN
;
Yan HUANG
;
Quan XIA
Author Information
1. 安徽医科大学药学院(合肥 230032)
- Keywords:
Curdione;
Deep venous thrombosis;
Fibrinolysis;
Antithrombosis;
D-dimer
- From:
China Pharmacist
2024;28(11):424-430
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether curdione inhibits the formation of deep vein thrombosis(DVT)in rats through thrombolytic effects.Methods Twenty-five rats were randomly divided into five groups,the normal control group,the sham operation group,the model group,the low dose group of curdione(60 mg/kg)and the high dose group of curdione(120 mg/kg),with five rats in each group.Using the inferior vena cava ligation model,blood was taken from the pulmonary aorta in a sodium citrate anticoagulant tube one week after drug administration.The coagulation and fibrinolytic indexes Fibrin Degradation Products,(FDP),D-dimer(D-D),plasminogen activator inhibitor(PAI)-1 and plasminogen(PLG)were detected in each group;the thrombus weight/weight-length ratio was detected;and the formation of venous thrombus in the lower limbs of rats was detected by hematoxylin-eosin(HE)staining nuclear factor;NF-κB protein expression in inferior vena cava tissues was dectected by Western Blot assay.Results There was no significant difference in plasma FDP levels among groups of rats(P＞0.05);compared with the model group,the plasma FDP and D-D levels of rats in each dosing group were increased,the plasma PAI-1 and PLG levels were elevated,and the fibrinolytic effect was better in the group with high dose of curdione administration than in the group with low dose of curdione administration(P＜0.05);compared with the model group,the thrombus weight/weight-length ratio,the thrombus mass and NF-κB protein expression in the curdione dosing group were decreased.Conclusion Curdione can inhibit the formation of DVT in rats by influencing the fibrinolytic regulators.
