Determination of three characteristic components of Ophiopogon japonicus and its adulterants in Shengmaiyin by UPLC-MS/MS
10.12173/j.issn.2097-4922.202403050
- VernacularTitle:UPLC-MS/MS测定生脉饮中麦冬及其掺伪品的3种特征成分
- Author:
Saiyan HUANG
1
;
Danyang XU
;
Yiran YAO
;
Shu WANG
;
Jie QIAN
;
Lin ZHU
;
Huanhuan LIU
Author Information
1. 南通市食品药品监督检验中心(江苏南通 226014)
- Keywords:
Shengmaiyin;
Ophiopogon japonicus;
Methylophiopogonanone A;
Liriopeside B;
Liriope muscari baily saponin C;
Ultra performance liquid chromatography tandem mass spectrometry
- From:
China Pharmacist
2024;28(11):390-396
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the quality and authenticity of Ophiopogon japonicus in Shengmaiyin,establish ultra performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS)for the determination of three characteristic components(methylophiopogonanone A,liriopeside B and liriope muscari baily saponin C),and provide technical support for drug supervision.Methods Samples were analyzed on a Phenomenex Kinetex F5 C18 column(100 mm×3.0 mm,2.6 μm)in a gradient elution mode with 0.1％formic acid water and-0.1％formic acid acetonitrile as the mobile phase at a flow rate of 0.4 mL/min,the column temperature was 30 ℃,and the injection volume was 1 μL.Mass spectrometer detector,electro spray ion source of positive and negative ions,and multi-reaction monitoring mode were used.Results The linear ranges of methylophiopogonanone A,liriopeside B and liriope muscari baily saponin C were 0.016 7-1.666 0,0.039 7-15.872 0 and 0.022 5～8.988 0 ng(r＞0.999 9),respectively.The average recovery rates of these three components were 85.16％,86.95％and 95.07％,respectively,with RSSDs of 2.65％,1.45％and 1.14％(n=6).The content of methylophiopogonanone A in thirty-eight batches was quite different.Seven batches of liriopeside B or liriope muscari baily saponin C were detected.Conclusion The method is simple and sensitive,and suitable for determining of three characteristic components in Shengmaiyin,which provides references for the quality control of Ophiopogon japonicus in Shengmaiyin.
