Pharmacognostical identification research of Inonotus obliquus
10.12173/j.issn.1008-049X.202312062
- VernacularTitle:桦褐孔菌的生药学鉴别研究
- Author:
Tianmi ZHU
1
;
Yuqing DUAN
;
Simeng WANG
;
Shuhe CHEN
;
Ying YANG
;
Keli CHEN
Author Information
1. 湖北省中医院/湖北中医药大学附属医院/湖北省中医药研究院药事部(武汉 430061)
- Keywords:
Inonotus obliquus;
Medicinal parts;
Basal source investigation;
Trait identification;
Microscopic identification;
Molecular biology;
High-performance liquid chromatography;
Content determination
- From:
China Pharmacist
2024;27(7):1108-1114
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the identification methods and traits of the Chinese medicinal materials Inonotus obliquus.Methods Samples were collected from the origin of northeast China,and source identification,molecular biology identification,trait identification,microscopic identification and HPLC assay were used to study the Inonotus obliquus,its non-pure fungous part,and counterfeit.Results Inonotus obliquus had a nodular shape,the microstructure was closely interconnected with mycelia,and the medicinal part was the sclerotium,not the"fruiting body"as stipulated in the current standards of traditional Chinese medicinal materials.Occasionally,a very small amount of tubule and subiculum could be seen in Inonotus obliquus.Tubule hole shape was circular,with a diameter of 130-190 μm,and ruptured.The pipe wall structure was special,which was composed of longitudinal arranged small compartment with diameter of 2-5-8 μm.Non-pure fungous part was the decayed host xylem infiltrated by mycelia.The contents of trametenolic acid and ergosterol in this part were significantly lower than the pure sclerotium(P<0.05).The microstructure and HPLC fingerprints of Inonotus obliquus and the counterfeit burl were clearly distinguishable from each other.Conclusion The method used is able to identify the authenticity of Inonotus obliquus and the quality of the medicinal materials.
