HPLC fingerprint and three main components determination of Modified Zengye Decoction
10.12173/j.issn.1008-049X.202311200
- VernacularTitle:加味增液汤HPLC指纹图谱及3种主要成分的含量测定
- Author:
Shiqi LIU
1
,
2
;
Chuang WU
;
Zhimin ZHU
;
Feng XU
;
Yaowen CHANG
;
Haiyan ZHANG
;
Xiaojun GOU
Author Information
1. 上海中医药大学附属宝山医院肛肠科(上海 201999)
2. 上海市宝山区中西医结合医院中心实验室(上海 201999)
- Keywords:
Modified Zengye Decoction;
Ferulic acid;
Aurantiamarin;
Harpagoside;
High performance liquid chromatography;
Fingerprint;
Content determination;
Quality control
- From:
China Pharmacist
2024;27(6):928-936
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for HPLC fingerprint analysis and determine three main components of Modified Zengye Decoction.Methods The chromatographic column was Shimadzu WondaSil C18 column(250 mm×4.6 mm,5 μm),the mobile phase was acetonitrile-0.3％aqueous phosphoric acid with a gradient elution procedure,the volume flow rate was 1.0 mL/min,the detection wavelengths were 265,203,310 and 290 nm,the column temperature was 25 ℃,and the injection volume was 20 μL.The HPLC fingerprints of the 10 batches of Modified Zengye Decoction were established,and the similarity analysis was performed by using the similarity evaluation system of chromatographic fingerprint of traditional Chinese medicine(version 2012A).The common peaks were identified and assigned,and the contents of the three main components were quantitatively determined.Results There were 17 common peaks in the fingerprints of 10 batches of Modified Zengye Decoction with similarities ranging from 0.872-0.989.The fingerprints recognized peak 9,14 and 17 as ferulic acid,aurantiamarin and harpagoside,respectively.The contents of ferulic acid,aurantiamarin and harpagoside were 0.067 3-0.174 8,0.498 8-1.522 7,0.270 9-0.802 4 mg/g,and the transfer rate were 30.74％-55.63％,11.77％-35.94％,23.15％-68.56％,respectively.Conclusion The established HPLC fingerprint analysis method combined with main components quantitative analysis method can be used for the quality analysis and control of Modified Zengye Decoction with simple analysis method and reliable results.
