Characteristic spectrum of Rheum tanguticum Maxim,ex Balf.water decoction and analysis of different origins and different processed products
10.19778/j.chp.2024.04.002
- VernacularTitle:唐古特大黄标准汤剂特征图谱研究以及不同基原、炮制品对比分析
- Author:
Kaiwei HUANG
1
,
2
,
3
;
Hui ZHANG
;
Shuping XU
;
Huiling YANG
;
Yanghua LI
;
Weizhi ZHAO
;
Zhijun GUO
;
Pei TAN
Author Information
1. 华润三九现代中药制药有限公司,广东惠州 516000
2. 安徽华润金蟾药业股份有限公司中药配方颗粒安徽省重点实验室,安徽淮北 235000
3. 华润三九医药股份有限公司,广东 深圳 518000
- Keywords:
Rheum tanguticum Maxim.ex Balf.;
ultra high liquid chromatography;
water decoction;
components;
quality study;
different origins;
different processed products
- From:
Drug Standards of China
2024;25(4):330-338
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an ultra-high liquid chromatography(UPLC)characteristic spectrum of Rheum tanguticum Maxim.ex Balf.water decoction and conduct chemical pattern recognition analysis,and to identify the medicinal materials of different origins and different processed products.Methods:UPLC was adopted to establish the characteristic spectra of 15 batches of Rheum tanguticum Maxim.ex Balf.Cluster analysis combined with principal component analysis was used to analyze their quality.Rhei Radix et Rhizoma from different origins and different processed products of Rheum tanguticum Maxim.ex Balf.were identified.Results:The characteristic spectrum of Rheum tanguticum Maxim.ex Balf.water decoction was established,18 common peaks were identi-fied,and 15 batches of Rheum tanguticum Maxim.ex Balf.were divided into 2 categories according to their origins by cluster analysis.The similarity between 15 batches of samples from different origins and the control spectrum was greater than 0.900.According to OPLS-DA analysis,a total of 6 markers(rhein-8-O-β-D-glu-cosid,resveratrol-4'-O-β-D-(6''-O-D-gallyl)glucopyranside,isolindleyin,rhein,epicatechin-3-O-D-gallate,and catechin)affecting the quality of Rheum tanguticum Maxim.ex Balf.water decoction samples were found.Rhei Radix et Rhizoma from different origins and different processed products of Rheum tanguticum Maxim.ex Balf.can be effectively distinguished.Conclusion:The established characteristic spectrum method is easy to operate and has good repeatability.It can be used for the quality control of Rheum tanguticum Maxim.ex Balf.water decoction,and can provide reference for the formulation of quality standard of formula granules of Rhei Radix et Rhizoma.
