Multiplex PCR identification of Zaocys dhumnades formula granules and three common counterfeit products
10.19778/j.chp.2024.04.001
- VernacularTitle:乌梢蛇配方颗粒与常见三种伪品的多重PCR鉴别研究
- Author:
Ye SONG
1
;
Yuqin LUO
;
Guowei LI
;
Leyao XIAN
;
Siyin TAN
;
Yaoyao FAN
;
Yijing LUO
;
Xiangdong CHEN
;
Dongmei SUN
Author Information
1. 广东一方制药有限公司,广东省中药配方颗粒企业重点实验室,广东佛山 528244
- Keywords:
Zaocys dhumnades;
Elaphe carinata;
Elaphe meollendorffi;
Ptyas korros;
formula granules;
multiplex allele-specific polymerase chain reaction
- From:
Drug Standards of China
2024;25(4):321-329
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a polymerase chain reaction(PCR)method to accurately discriminate the crude materials and aqueous extract of Zaocys dhumnades,Elaphe carinata,Elaphe meollendorff and Ptyas korros.Methods:Specific primers were designed using mitochondrial Cytb gene(CO1)as a target gene,and annealing temperature,number of cycles and the type of DNA polymerases were optimized.The mixed samples were detected by this method.Results:By this multiplex allele-specific PCR identification method,135,182,246 and 197 bp of specific fragments were amplified from DNA templates of Zaocys dhumnades,Elaphe carinata,Elaphe meollendorffi and Ptyas korros,respectively,following the conditions:cycle number of 35,annealing temperature of 62 ℃.The adulterants and the blank control showed no bands.The method could simultaneously and accurately identify the snake-derived components in the mixed samples.Conclusion:The method can be used to identify the samples of Zaocys dhumnades,Elaphe carinata,Elaphe meollendorffi and Ptyas korros simultaneously,accurately and rapidly,and is suitable for the identification of standard decoctiond and formula granules samples.
