Effect of different pretreatment methods on the detection of pesticide residues in five traditional Chinese medicines
10.19778/j.chp.2024.03.006
- VernacularTitle:不同前处理方法对5种中药农药残留量检测的影响
- Author:
Wanqing ZHANG
1
;
Yuanxi LIU
;
Gaopian CHEN
;
Huiru ZHU
;
Yufang ZHU
;
Xiaoxiao LIU
Author Information
1. 广东省药品检验所,广州 510663
- Keywords:
pesticide residues;
QuEChERS;
HLB material;
Angelicae Sinensis Radix;
ChrysanthemI Flos;
Lych Fructus;
Astragali Radix;
Lonicerae Japonicae Flos
- From:
Drug Standards of China
2024;25(3):251-256
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study effects of different pretreatment methods on the detection of pesticide residues in Angelicae Sinensis Radix,ChrysanthemI Flos,Lych Fructus,Astragali Radix and Lonicerae Japonicae Flos.Methods:The samples were treated with QuEChERS method and high-speed homogenization combined with hydro-phile-lipophile balance(HLB)solid-phase extraction method,and the residual amounts of carbofuran,3-carboxyl-carbofuran,phorate,phorate sulfone,phorate sulfoxide,and methyl isoflurophos were simultaneously determined using UHPLC-MS/MS.With a gradient elutionof 0.1％formic acid solution containing 5 mmol·L-1 ammonium formate and t 0.1％formic acid solution containing 5 mmol·L-1 ammonium formate-acetonitrile(volume ratio of 5:95),Ultra-performance liquid chromatography column Agilent Poroshell 120 SB-C18(2.1 mm × 100 mm,2.7μm)was used at 35 ℃ and the electrospray ion source was scanned in the positive ion mode and detected in the multiple reaction monitoring mode in mass spectrometry.Results:The deviation of the results measured by QuECh-ERS method and HLB solid-phase extraction method was between 9.09％-55.56％.Conclusion:In the selection of the pretreatment method for the detection of pesticide residues in traditional Chinese medicine,it is recommen-ded to take the measurement data of positive samples as the evaluation index and basis,and choose the method with higher measurement value and high extraction efficiency.
