Determination of two components and salicylic acid in aspirin and dipyridamole tablets by HPLC-DAD wavelength switching method
10.19778/j.chp.2024.02.009
- VernacularTitle:HPLC-DAD波长切换法测定阿司匹林双嘧达莫片的含量及游离水杨酸
- Author:
Haiyan TIAN
1
;
Xiangguo LIU
Author Information
1. 德州市食品药品检验检测中心,山东德州 253015
- Keywords:
aspirin;
dipyridamole;
free salicylic acid;
content determination;
content uniformity;
HPLC-DAD wavelength switching method
- From:
Drug Standards of China
2024;25(2):164-167
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an HPLC-DAD wavelength-switching method for the determination of aspirin dipyridamole tablets and free salicylic acid.Methods:An Agilent ZORBAX SB C18 column(250 mm ×4.6 mm,5 μm)was adopted with acetonitrile-tetrahydrofuran-glacial acetic acid-water(20∶5∶5∶70)as the mobile phase,and isocratic elution was performed at the flow rate of 1.0 mL·min-1,the column temperature of 30 ℃,and the detection wavelengths were 276 nm for aspirin,284 nm for dipyridamole and 303 nm for salicylic acid.Results:Aspirin,dipyridamole and free salicylic acid had a good linear relationship in the range of 38.37-383.71,9.60-96.05 and 1.02-10.21 μg·mL-1 respectively.The average recoveries were 99.88％(RSD=0.23％),99.01％(RSD=0.46％)and 100.99％(RSD=0.75％),respectively.There was no signif-icant difference for the content resuls between the established method and the standard method.The content uni-formity of A+2.2S ranges from 4.7 to 6.4.Conclusion:The established method is simple and suitable for the quality control of aspirin dipyridamole tablets.
