Investigation and determination of relative correction factor of pre-vitamin D
10.19778/j.chp.2024.02.006
- VernacularTitle:前维生素D相对校正因子的研究及测定
- Author:
Jieming SHI
1
;
Cheng WANG
;
Liwen ZHANG
;
Shunling DONG
;
Jian LE
;
Song YUAN
;
Yihong LU
;
Dandan WANG
;
Wankui XU
;
Shufeng ZHENG
Author Information
1. 广州市药品检验所,广州 510160
- Keywords:
vitamin D;
pre-vitamin D;
relative correction factor;
influencing factor;
HPLC;
external standard method
- From:
Drug Standards of China
2024;25(2):147-153
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To determine the relative correction factor of pre-vitamin D and simplify the calculation method of vitamin D assay.Methods:By studying the calculation method of vitamin D content in drug standards of various countries,HPLC was used to determine the relative correction factor of pre-vitamin D,and the influencing factors of determination were investigated.Results:The relative correction factors of pre-vitamin D at 254 nm and 265nm wavelength were determined by statistical analysis of 7 laboratories in China.Conclusion:Using the pre-vi-tamin D relative correction factor method to calculate the total amount of vitamin D simplified the experimental steps can be simplified by the pre-vitamin D relative correction factor method to calculate the total amount of vitamin D and the random operating errors can be avoided.The method is rapid and accurate,and lay a solid foundation for further improving the standard of vitamin D preparations.
