Establishment of suckling rabbits model for monkeypox virus infection
10.3867/j.issn.1000-3002.2024.11.004
- VernacularTitle:猴痘病毒感染乳兔模型的建立
- Author:
Gaojie SONG
1
;
Lingling CHENG
;
Dapeng LI
;
Xue LI
;
Chengyuan MA
;
Xianyan SUN
Author Information
1. 九江学院基础医学院细胞精准治疗江西省重点实验室,江西 九江 332005
- Keywords:
monkeypox virus;
animal model;
suckling rabbits;
pathological changes
- From:
Chinese Journal of Pharmacology and Toxicology
2024;38(11):834-838
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To establish a model of monkeypox virus(MPXV)infection in suckling rabbits and analyze its pathological changes.METHODS Ten-day-old suckling rabbits were divided into two groups:the MPXV group(intranasally inoculated with 100 μL of MPXV at 106 PFU·mL-1)and the normal control group(intranasally inoculated with 100 μL of PBS solution),with 10 rabbits in each group.After infection,such indicators as body weight,survival rate,and mental state of the suckling rabbits were monitored daily for 15 days.On days 3,9,and 15 post-infection,samples of the heart,liver,spleen,lung,kidney,brain,small intestine,and peripheral blood were collected from each group for RT-qPCR to detect viral the load.Histopathological analysis using HE staining was performed on samples collected on day 9 post-infection.RESULTS Suckling rabbits infected with MPXV exhibited weight loss,reduced survival rates,and such symptoms as lethargy and chilliness,which were similar to the clinical manifes-tations observed in humans infected with MPXV.Viral load results indicated the presence of the virus in the heart,liver,spleen,lung,kidney,brain,small intestine,and peripheral blood,with varying degrees of pathological changes in each tissue and organ.CONCLUSION Intranasal inoculation can help estab-lish a model of MPXV infection in suckling rabbits.
