Research progress in and prospects of humanized liver chimeric mouse models
10.3867/j.issn.1000-3002.2024.07.009
- VernacularTitle:人源化肝嵌合小鼠模型的研究进展与展望
- Author:
Yahong ZHANG
1
;
Qi ZHANG
;
Cai ZHANG
;
Yuan WEI
Author Information
1. 江苏大学药学院,江苏镇江 212013
- Keywords:
humanized liver chimeric mouse;
immunodeficiency;
drug metabolism;
liver diseases;
drug screening
- From:
Chinese Journal of Pharmacology and Toxicology
2024;38(7):550-558
- CountryChina
- Language:Chinese
-
Abstract:
Research into human drug metabolism,liver disease modeling as well as drug screening heavily depend on cell cultures for in vitro validation and experimental animal models for in vivo analysis.However,these methods are not without limitations.Cell stability is often compromised in vitro,and the species-specific differences between experimental animals and humans restrict the accuracy of these models in replicating in vivo drug metabolism and the pathophysiological features of human liver diseases.Humanized liver chimeric mouse models,developed through the transplantation and expan-sion of human liver cells into the livers of live mice,replicate the specific functionalities of the human liver.This replication is instrumental in fulfilling a variety of research objectives,including the replication of hepatitis viruses within mice,the modeling of liver metabolic disorders,the screening of liver cell-targeted therapeutic agents,and the evaluation of drug-induced hepatotoxicity.As such,these models are regarded as optimal for the preclinical validation of drug efficacy and safety assessment.This paper presents a comprehensive review and a vision of three representative humanized liver chimeric mouse models in terms of classification,construction principles,limitations,and current applications,thereby offering reference for the selection of appropriate models in research.
