Ability of iron sulfur cluster binding protein 1 to respond to magnetic fields
10.3867/j.issn.1000-3002.2024.06.003
- VernacularTitle:铁硫簇结合蛋白1的磁感应能力
- Author:
Mengnan LIU
1
;
Xiaoyu TIAN
;
Wencan FANG
;
Ning WU
;
Jin LI
;
Hong LI
Author Information
1. 军事医学研究院国家安全特需药品全国重点实验室,北京 100850
- Keywords:
iron sulfur cluster binding protein 1;
cryptochrome;
magnetic receptor protein;
magnet-oception;
magnetogenetics
- From:
Chinese Journal of Pharmacology and Toxicology
2024;38(6):420-425
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the effect of iron sulfur cluster binding protein 1(ISCA1)mag-netic field on calcium inflow.METHODS ① A plasmid containing the ISCA1、Magneto 2.0 gene sequence,and an empty plasmid was prepared for lentivirus packaging,with all the three plasmids carrying the mCherry gene.The infection efficiency of lentiviruses was assessed using fluorescence microscopy.② The immunoprecipitation technique was employed to ascertain the interactions between ISCA1 and cryptochrome(CRY1)as well as CRY2 proteins.③ The technique of live cell calcium imaging was used to detect the intracellular calcium influx in cells exhibiting high levels of ISCA1 or Magneto 2.0 expression under the influence of a magnetic field(40 mT,0.1Hz,90%duty cycle).RESULTS ① Red fluorescence was observed by fluorescence microscopy,indicating successful transfection of lentivirus.② The exogenous ISCA1 proteins exhibited no affinity towards the endogenous CRY1 or CRY2 proteins.③ The green fluorescence intensity of the Magneto 2.0 group increased by(1.8±0.5)times compared to the pre-magnetic addition level,indicating a significant influx of calcium ions.However,there was no significant difference in the green fluorescence intensity between the ISCA1 group and control group before and after magnetic addition.CONCLUSION Cells with exogenously high expres-sion of ISCA1 do not elicit calcium influx and exhibit no discernible magnetic induction following stimula-tion by this magnetic field.
