Study on inhibitory effect of alisol B on non-small cell lung cancer based on network pharmacology and its mechanism
- VernacularTitle:基于网络药理学研究泽泻醇B抑制非小细胞肺癌的作用及机制
- Author:
Liu-Yan XIANG
1
;
Wen-Xuan WANG
;
Si-Meng GU
;
Xiao-Qian ZHANG
;
Lu-Yao LI
;
Yu-Qian LI
;
Yuan-Ru WANG
;
Qi-Qi LEI
;
Xue YANG
;
Ya-Jun CAO
;
Xue-Jun LI
Author Information
- Keywords: alisol B; NSCLC; natural medicine; net-work pharmacology; tumor microenvironment; tumor-associated macrophages
- From: Chinese Pharmacological Bulletin 2024;40(12):2375-2384
- CountryChina
- Language:Chinese
- Abstract: Aim To explore the potential genes and mechanism of alisol B in the treatment of non-small cell lung cancer(NSCLC).Methods The proliferation and migration of NSCLC cells were detected by CCK-8 and Transwell.Genes of NSCLC and alisol B were col-lected through TCGA and compound gene prediction database,and their intersection genes were obtained.The network of protein-protein interaction(PPI)was constructed by using String database,and the top 20 key nodes were screened out,and the prognosis-related proteins related to the prognosis of NSCLC were screened out by using R language,and the intersection of them was obtained.The potential mechanism of ali-sol B on NSCLC was explored by KEGG and GO en-richment analysis and the relationship between related genes and immune cells,which was verified by cell-lev-el experiments.Results Alisol B inhibited the cell activity and migration ability of NSCLC cells.Five im-portant genes were identified by network pharmacologi-cal analysis:CCNE1,CDK1,COL1A1,COL1A2 and COL3A1.The results of cell experiment showed that al-isol B down-regulated the expression of Cyclin E1,CDK1 and COL1A2 in NSCLC cells.In addition,alisol B could inhibit the expression of COL1A2 and M2 macrophage marker CD206 in macrophages.Conclu-sions Alisol B may inhibit the proliferation of tumor cells by down-regulating CDK1 and Cyclin E1,and may affect the function of macrophages by inhibiting COL1A2,thus regulating the tumor immune microenvi-ronment and inhibiting NSCLC.
