Exploring the effects of sirolimus on the growth and development of zebrafish embryo models
- VernacularTitle:探索西罗莫司对斑马鱼胚胎模型生长发育的影响
- Author:
Zi-Xin ZHANG
1
;
Tong QIU
;
Jiang-Yuan ZHOU
;
Xue-Peng ZHANG
;
Xue GONG
;
Kai-Ying YANG
;
Yu-Ru LAN
;
Si-Yuan CHEN
;
Yi JI
Author Information
- Keywords: sirolimus; growth and development; toxic-ity; zebrafish; embryo; blood concentration
- From: Chinese Pharmacological Bulletin 2024;40(12):2368-2374
- CountryChina
- Language:Chinese
- Abstract: Aim To explore the effects of sirolimus on the growth and development of motor,vascular,nerv-ous,and immune systems through zebrafish models.Methods After 3 hours of fertilization of zebrafish embryos,different concentrations of sirolimus were add-ed to the growth environment,and the growth and de-velopment of the embryos was recorded.Transgenic ze-brafish models labeled with blood vessels,nerves or im-mune cells were used to compare the drug effects on the growth and development of those systems.Results At the concentration of 0.5 μmol·L-1,the hatching rate and the body length(P＜0.01)were significantly smaller than those of the control group,and movement was also significantly slowed down.Meanwhile,the length of axons of the nervous system,the development of intersegmental vessels,and the growth of immune cells were significantly delayed by drug treatment.But when the concentration was below 0.1 μmol·L-1,there was no statistically difference between the control group and the sirolimus group.Conclusions When the concentration of sirolimus exceeds a certain level,it can significantly slow down the growth and development of movement,blood vessels,nervous system and im-mune system of zebrafish.Therefore,in clinical prac-tice,it is important to monitor the blood concentration of sirolimus in children on time.
