Effect of salidroside combined with rosavin on ischemic brain injury in rats
- VernacularTitle:红景天苷联合洛塞维对缺血性脑卒中大鼠的作用
- Author:
Wen-Fang LAI
1
;
Yu-Ting JIANG
;
Hui-Ling WU
;
Qing-Qing WU
;
Jing-Quan CHEN
;
Xue-Rui ZHENG
;
Gui-Zhu HONG
Author Information
- Keywords: salidroside; rosavin; ischemic stroke; Ne-uN; primary nerve cells; TGF-β1
- From: Chinese Pharmacological Bulletin 2024;40(12):2303-2311
- CountryChina
- Language:Chinese
- Abstract: Aim To study the effect of salidroside combined with rosavin on ischemic stroke in rats.Methods The model of MCAO was established by u-sing thread-embolic method.The rats were divided into the sham group,MCAO group,salidroside combined with rosavin group,positive control group,and the drug was given continuously for seven days.The infarct volume was measured by MRI and neurological deficit score was evaluated by Zea-Longa.The levels of Ne-uN,BDNF,TGF-β1,p-Smad were observed by West-ern blot and immunofluorescence staining.The expres-sions of IL-1β,TNF-α and IL-6 were performed by RT-qPCR/ELISA.The primary cortical neurons were isolated,OGD/R inducted,divided into the normal group,OGD/R group,salidroside combined with rosa-vin group,and TGF-β1 inhibitor+salidroside com-bined with rosavin group,the drug was given for 24 hours,and the expressions of NeuN,BDNF,IL-1β,TNF-α and IL-6 were measured.Results Salidroside combined with rosavin could decrease the infarct vol-ume,improve the neurological function,promote the levels of Neun,BDNF,TGF-β1,p-Smad,and inhibit the expressions of IL-1β,TNF-α and IL-6.Salidroside combined with rosavin could promote NeuN,BDNF,inhibit IL-1β,TNF-α,IL-6 in primary nerve cells in-duced by OGD/R,and these effects were blocked by TGF-β1 inhibitor.Conclusions Salidroside combined with rosavin has neuroprotective effects on MCAO rats,and primary neurons are induced by OGD/R,and these effects are closely related to the TGF-β pathway.
