Effects of astragalus angelica on apoptosis and expression of Bax and caspase-3/9 in rabbit chondrocytes after fresh osteochondral allograft
- VernacularTitle:黄芪-当归对新鲜同种异体骨软骨移植术后兔软骨细胞凋亡及Bax、caspase-3/9表达的影响
- Author:
Wan-Tao DONG
1
,
2
;
Pan YANG
;
Xiu-Juan YANG
;
Shi-Ming QIU
;
Peng YUAN
;
Jing-Yi LIU
;
Jiu-Mei HUANG
;
Yu ZHOU
Author Information
- Keywords: Astragalus Angelica drug pair; fresh osteo-chondral allograft; type Ⅱ collagen; Bax; caspase-3; caspase-9
- From: Chinese Pharmacological Bulletin 2024;40(12):2288-2294
- CountryChina
- Language:Chinese
- Abstract: Aim To observe the effect of Astragalus membranaceus and Angelica sinensis on the apoptosis of chondrocytes,and to investigate the effect of Astrag-alus membranaceus and Angelica sinensis on the sur-vival of fresh ostecartilage allograft.Methods Forty-eight 4-month-old New Zealand white rabbits,half male and half female,were randomly divided into sham operation group,model group,positive group and As-tragalus and Angelica 5∶1 group.In addition to the sham operation group,the other groups were both male and female donors and recipients for knee joint osteo-cartilage cross transplantation modeling.After 8 weeks of drug intervention,samples were taken for general observation,HE staining,saffrane-O staining,immu-nohistochemical staining,qPCR and Western blot de-tection.Results Compared with model group,As-tragalus and Angelica 5∶1 group and positive group,the repair site healed better,the morphology of osteo-chondrocytes tended to be normal,and the division and proliferation were obvious.Proteoglycan deposition in-creased and type Ⅱ collagen content was higher,the differences were statistically significant(P＜0.05).qPCR and Western blot results showed that compared with model group,the mRNA and protein expressions of Bax,caspase-3 and caspase-9 in other groups were significantly decreased(P＜0.05).Conclusion As-tragalus and Angelica can promote the survival of fresh osteochondral allograft,and its mechanism may be re-lated to promoting collagen production,promoting chondrocyte proliferation and inhibiting chondrocyte apoptosis.
