The protective effect of licorice on cisplatin-induced liver injury in rats based on untargeted metabolomics study
- VernacularTitle:基于非靶向代谢组学研究甘草对顺铂诱导肝损伤大鼠的保护作用
- Author:
Ting-Mei YIN
1
;
Bi-Qian YANG
;
Guang-Miao GAO
;
Xiao-Yan FU
;
Xiao-Long LIAN
;
Ling-Ling YANG
;
Jie LI
;
Yi DENG
Author Information
- Keywords: licorice; cisplatin; liver injury; mecha-nism of action; metabolomics; amino acid metabolism
- From: Chinese Pharmacological Bulletin 2024;40(12):2246-2255
- CountryChina
- Language:Chinese
- Abstract: Aim To study the mechanism of action of licorice in alleviating cisplatin liver injury.Methods Forty-eight SD rats were randomly divided into a blank group,a model group,a positive control group and lico-rice administration groups(450,900 and 1 800 mg·kg-1).After 5 days of prophylactic administration,8 mg·kg-1 of cisplatin was injected intraperitoneally in-to the model,positive control,and licorice administra-tion groups to establish an acute liver injury model.LC-MS/MS untargeted metabolomics was used to ana-lyze the differential metabolites and metabolic pathways of licorice to alleviate cisplatin acute liver injury.Re-sults PLS-DA score plots showed significant separa-tion of metabolomics samples.The analysis yielded 119 differential metabolites associated with cisplatin liver injury,of which 31 differential metabolites were signifi-cantly regressed after licorice intervention and were mainly involved in D-arginine and D-ornithine metabo-lism;parathyroid hormone synthesis,secretion,and ac-tion;tyrosine metabolism;biosynthesis of phenylala-nine,tyrosine,and tryptophan;β-alanine metabolism;and amino acid and nucleotide sugar metabolism.Con-clusions Metabolomics analysis indicates that licorice can alter the metabolic profile of cisplatin-induced he-patic injury rats,and its mechanism of action may be related to its improvement of the levels of differential metabolites and its involvement in the regulation of a-mino acid metabolism and other related pathways.
