Schisandrol B protects intestinal cells against irradiation-induced injury via Nrf2-GPX4 signaling pathway
- VernacularTitle:五味子醇乙经Nrf2-GPX4信号通路防治肠道辐照损伤
- Author:
Ze-Kun WU
1
,
2
;
Chang-Kun HU
;
Liang-Liang ZHANG
;
Ze-Bin LIAO
;
Yue GAO
Author Information
- Keywords: schisandrol B; intestinal injury; ionizing radiation; Nrf2; ferroptosis; inflammation
- From: Chinese Pharmacological Bulletin 2024;40(12):2236-2246
- CountryChina
- Language:Chinese
- Abstract: Aim To investigate the radio-protective effect of schisandrol B(Sol B)underlying mechanism in alleviating gastrointestinal(GI)toxicity and intesti-nal damage induced by irradiation(IR)exposure dur-ing radiotherapy against abdominal and pelvic malig-nancies or the nuclear accident.Methods The mouse intestinal injury model was established through 4Gy ir-radiation.Immunohistochemistry,immunofluorescence staining,ELISA were used to measure DNA damage,apoptosis,inflammatory reaction,and oxidative stress in the intestine of mice after irradiation.The protective effect of Sol B on intestines injury was evaluated,and the mechanism of the related oxidative stress and in-flammatory response pathway was discussed.Results Sol B significantly improved the survival rates of intes-tinal cells upon IR exposure.The accumulation of DNA damage,apoptosis rate,and inflammatory factors in intestinal tissues were significantly inhibited.The decreased levels of ZO-1 and occludin induced IR inju-ry were rescued by Sol B,indicating the improvement of intestinal structure.The radio-protective activities of Sol B were involved in the stimulation of Nrf2 and the subsequent ferroptosis surveillance.Conclusion Sol B demonstrates a promising agent to ease intestinal in-jury during radiotherapy or nuclear accidents,in which GPX4 related ferroptosis surveillance mediated by Nrf2 is involved in the radio-protective effect of Sol B.
