Study on pathogenesis of PMDD liver-qi reversal syndrome mediated by GABAARsubunit in amygdala and hippocampus of rats based on tetrahydroprogesterone
- VernacularTitle:基于四氢孕酮介导大鼠杏仁核和海马脑区GABAAR亚基的经前烦躁障碍症肝气逆证发病机制研究
- Author:
Yu-Chen QI
1
;
Dong-Mei GAO
;
Ya SUN
;
Tian-Tian GAO
;
Qi SHEN
;
Wei-Lin CUI
;
Feng-Qin WEI
;
Xiao-Li SONG
;
Jie-Qiong WANG
Author Information
- Keywords: PMDD liver-qi inverse syndrome; recep-tive and non-receptive phases; ALLO; GABAARα4 and δ subunits; amygdala and hippocampus; mecha-nism study
- From: Chinese Pharmacological Bulletin 2024;40(11):2131-2140
- CountryChina
- Language:Chinese
- Abstract: Aim To observe the behavioral effects of exogenous allopregnanolone(ALLO)and its inhibitor finasteride on the receptive period(R)and non-recep-tive period(NR)of PMDD liver-qi inversion model rats and the expression of GABAARα4,GABAARδ mR-NA and protein effects to explore its pathogenesis.Methods The PMDD liver-qi reverse syndrome rat model was prepared.The rats were divided into the normal group R and NR(control-R,control-NR),model group R and NR(Model-R,Model-NR),nor-mal group R+ALLO and NR+ALLO(Control+A-R,Control+A-NR),and model group R+ALLO and NR+ALLO(Model+A-R,Model+A-NR),model group R+finasteride and NR+finasteride(Model+F-R,Model+F-NR).The elevated cross labyrinth ex-periment and social interaction experiment were used to detect the behaviors of rats;fluorescence quantitative PCR and immunofluorescence were used to detect the expression of GABAARα4 and 8 mRNA and protein in rat amygdala and hippocampus.Results In the be-havioral evaluation,in the NR period,in the elevated cross maze test and in the social interaction test,the rats in the model group had anxiety behavior and de-creased social communication ability(P＜0.05),while the rats in the Model+A group could effectively relieve anxiety symptoms and improve their social com-munication ability(P＜0.05),and the rats in the Model+F group had increased anxiety behavior and social disorder(P＜0.05).In fluorescence quantita-tive PCR and immunofluorescence experiments,the ex-pression of GABAARα4 subunit in the model group was up-regulated in the hippocampus(P＜0.01),and the expression of δ subunit was down-regulated(P＜0.01);the expression of GABAARα4 subunit in the a-mygdala and hippocampus of the Model+A group de-creased(P＜0.01),and the expression of δ subunit increased in the hippocampus(P＜0.01).Conclu-sions The abnormal expression of GABAARα4 and 8 subunits mediated by ALLO improves the anxiety symptoms and social interaction ability of PMDD,which is the pathogenesis of PMDD liver-qi reverse syndrome,and provides basis and support for subse-quent exploration of the pathogenesis of PMDD liver-qi reverse syndrome.
