Effect of berberine on regulating NF-κB p65/TGF-β1/CTGF signaling pathway in reducing renal fibrosis injury in mice
10.12360/CPB202403059
- VernacularTitle:小檗碱下调NF-κB p65/TGF-β1/CTGF信号通路缓解小鼠肾纤维化损伤
- Author:
Guang-Yao LI
1
;
Jia-Min LIANG
;
Meng-Tong JIN
;
Duan XI
;
Peng LIU
;
Peng WANG
;
Rui-Hua WANG
;
Qing-Qing LIU
Author Information
1. 临汾市中心医院,山西临汾 041000
- Keywords:
berberine;
UUO;
renal fibrosis;
renal inju-ry;
inflammation;
NF-κB p65/TGF-β1/CTGF signaling
- From:
Chinese Pharmacological Bulletin
2024;40(11):2042-2047
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the protective effect of berberine(BBR)on mice with unilateral ureteral obstr-uction(UUO)and explore its mechanism.Methods C57BL/6 mice were randomly divided into the sham group,UUO group,and BBR treatment groups(50,100 and 200 mg·kg-1),with eight mice in each group.Except the sham group,the other groups were subjected to left ureteral ligation to establish the UUO model.Af-ter modeling,the mice in the sham and UUO groups were fed normal saline,and the mice in the BBR treat-ment groups were fed(50,100,200)mg·kg-1 BBR by gavage for 14 days,respectively.Biochemical analy-zer was employed to detect the levels of serum creati-nine(Scr)and blood urea nitrogen(BUN).HE,Mas-son,TUNEL and immunohistochemical staining were used to observe the pathological changes of renal tis-sue.ELISA was employed to detect the expression of pro-inflammatory cytokines in renal tissue homogenate.Western blot was used to detect the protein levels of NF-κB p65,TGF-β1 and CTGF in mouse kidney.Re-sults Compared with the UUO group,the levels of Scr and BUN in the BBR group were significantly reduced.Renal injury and interstitial fibrosis were alleviated.The expression of pro-inflammatory cytokines decreased in kidney.The expression of NF-κB p65,TGF-β1 and CTGF decreased.All results showed some degree of dose dependence.Conclusion Berberine has a sig-nificant protective effect on unilateral ureteral obstruc-tion mice,and the mechanism may be that BBR has the potential to inhibit NF-κB p65/TGF-β1/CTGF signa-ling pathway,thus reducing renal inflammation and fi-brosis.
