Effects of butin on regulation of pyroptosis related proteins on proliferation,migration and cycle arrest of human rheumatoid arthritis synovial fibroblast
10.12360/CPB202402039
- VernacularTitle:漆黄素调控焦亡相关蛋白对人类风湿关节炎滑膜成纤维细胞增殖、迁移及周期阻滞影响
- Author:
Hao LI
1
;
Xue-Ming YAO
;
Xiao-Ling YAO
;
Hua-Yong LOU
;
Wei-Dong PAN
;
Wu-Kai MA
Author Information
1. 贵州中医药大学第二附属医院风湿免疫科
- Keywords:
butin;
rheumatoid arthritis;
cell cycle;
inflammatory factors;
pyroptosis mechanism;
ethno-medicine
- From:
Chinese Pharmacological Bulletin
2024;40(10):1937-1944
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the regulatory mecha-nism of butin on the proliferation,migration,cycle blockage and pyroptosis related inflammatory factors in human fibroblast-like synoviocytes of rheumatoid arthri-tis(HFLS-RA).Methods Cell proliferation,migra-tion and invasion were studied using cell migration and invasion assays.Cell cycle was detected by flow cytom-etry,and the expression of the pyroptosis-associated in-flammatory factors IL-1β,IL-18,caspase-1 and caspase-3 was detected by ELISA,RT-qPCR and West-ern blot.Results Migration and invasion experiments showed that the cell proliferation rate of the butin group was lower than that of the blank control group(P＜0.05).Cell cycle analysis demonstrated that in the G0/G1 phase,the DNA expression was elevated in the medium and high-dose groups of butin(P＜0.05),while in the G2 and S phases,the DNA expression was reduced in the medium and high-dose groups of butin(P＜0.05).The results of ELISA,RT-qPCR and Western blot assay revealed that the expression of IL-1β,IL-1 8,caspase-1,and caspase-3 decreased in the butin group compared with the IL-1β+caspase-3 in-hibitor group(P＜0.05).Conclusions Butin inhib-its HFLS-RA proliferation by inhibiting the synthesis of inflammatory vesicles by caspase-1 in the pyroptosis pathway,thereby reducing the production and release of inflammatory factors such as IL-1β and IL-18 down-stream of the pathway,and also inhibits HFLS-RA pro-liferation by exerting a significant blocking effect in the G1 phase,which may be one of the potential mecha-nisms of butin in the treatment of RA.
