mfat-1 gene therapy prevents and ameliorates multiple sclerosis in mice
- VernacularTitle:mfat-1基因疗法有效预防及治疗小鼠多发性硬化症
- Author:
Min-Yi TANG
1
;
Xin-Yun BI
;
Shuai WANG
;
Chao-Feng XING
;
Xiao-Li WU
;
Zi-Jian ZHAO
;
Fang-Hong LI
Author Information
- Keywords: mfat-1; gene therapy; ω-3 polyunsaturat-ed fatty acids; ω-6 polyunsaturated fatty acids; multi-ple sclerosis; experimental autoimmune encephalomye-litis
- From: Chinese Pharmacological Bulletin 2024;40(10):1930-1936
- CountryChina
- Language:Chinese
- Abstract: Aim To investigate the preventive and therapeutic effects of the mfat-1 gene therapy on exper-imental autoimmune encephalomyelitis in mice.Meth-ods mfat-1 gene therapy was used to render the host endogenous capability of producing ω-3 PUFAs,con-comitantly reduce the levels of ω-6 PUFAs,and change the proportion of ω-3/ω-6 PUFAs.Then,the levels of PUFAs in blood were analyzed by gas chromatography.The neurological deficits in mice were evaluated by neurological dysfunction score.HE staining and LFB staining of mouse spinal cord slices were used to ob-serve central nervous system inflammation infiltration and demyelinating lesions.Flow cytometry microsphere microarray technology was used to detect the content of cytokines in serum.Results The mfat-1 gene therapy could significantly raise the proportion of ω-3/ω-6 PU-FAs(P＜0.05),markedly delay the incubation period and peak period and reduce neurological dysfunction scores(P＜0.05),and improve inflammation and de-myelination of spinal cords(P＜0.05).It could also greatly increase the levels of IL-2,IFN-γ,IL-4 and IL-17 in serum(P＜0.05).Conclusion The pro-portion of ω-3/ω-6 PUFAs in blood circulation en-hanced by mfat-1 gene therapy can effectively prevent and treat experimental autoimmune encephalomyelitis in mice.
