Alvianolic acid B exerts a protective effect on random skin flaps through autophagy mediated by activation of TFE3
- VernacularTitle:丹酚酸B通过激活TFE3介导的自噬发挥对随机皮瓣的保护作用
- Author:
Zhong-Bing HAN
1
;
Hui-Wen YANG
;
Hai-Zhou NIU
;
Kuan-Kuan ZHANG
;
Yang-Yang LIU
;
Pan-Pan XU
;
Ling-Ti KONG
;
Chang-Chun ZHANG
Author Information
- Keywords: salvianolic acid B; random skin flap; TFE3; autophagy; ischemia-reperfusion injury; angio-genesis
- From: Chinese Pharmacological Bulletin 2024;40(10):1912-1920
- CountryChina
- Language:Chinese
- Abstract: Aim To observe the role of salvianolic acid B(Sal B)in enhancing the survival of random skin flaps and to preliminarily explore its potential mecha-nisms.Methods The appearance,degree of edema,color and hair condition of the skin flap were evaluated seven days after operation.The vascular network and blood flow of random flaps were measured by laser Doppler flow measurement.HE staining was used to detect the growth of microvessels in random flaps.The expressions of VEGF and CD34 were detected by im-munohistochemistry,the expressions of RIPK1,2 and LC3 Ⅱ were detected by immunofluorescence,and the effects of autophagy related proteins and signaling path-ways were detected by Western blot.Results The ex-perimental results showed that Sal B induced autophagy in the random skin flaps,promoted angiogenesis,and reduced oxidative stress and necrotic apoptosis,signifi-cantly increasing the survival rate of the flaps.Immu-nohistochemistry,immunofluorescence staining,and Western blot confirmed that Sal B induced autophagy in the random skin flaps by activating TFE3 protein.Conclusion Sal B can promote autophagy in cells of random skin flaps and reduce their necrotic apoptosis by activating TFE3 protein.
