Src kinase is involved in postoperative pain through enhancing GlyR-α3 phosphorylation
10.12360/CPB202403020
- VernacularTitle:Src激酶通过磷酸化GlyR-α3参与术后痛
- Author:
Li YANG
1
;
Hu-Hu BAI
;
Sha-Sha HE
;
Si-Jia XING
;
Cheng-Song LIU
Author Information
1. 甘肃中医药大学附属医院药学部
- Keywords:
Src;
GlyR-α3;
postoperative pain;
tyro-sine phosphorylation;
paw withdrawal threshold;
paw withdrawal latency
- From:
Chinese Pharmacological Bulletin
2024;40(10):1879-1883
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the role of GlyR-α3 in postoperative pain.Methods The model of postoper-ative pain was established by incision of mouse skin and muscle.Pain responses including paw withdrawal thresholds(PWTs),paw withdrawal latencies(PWLs)and licking toes time were conducted and recorded.Protein contents and tyrosine phosphorylation levels of GlyR-α3 were detected by co-immunoprecipitation and immunoblotting.Bosutinib was used to inhibit Src ki-nase activity to verify whether Src was involved in tyro-sine phosphorylation of GlyR-α3.Results The PWTs and PWLs in postoperative pain model mice were sig-nificantly reduced,whereas the licking time increased significantly.The tyrosine phosphorylation level of GlyR-α3 in the dorsal root ganglia(DRG)from inci-sion sidesignificantly increased compared with contra-lateral side,while the protein expression showed no sta-tistical significance.The expression of Src in DRGin-creased after operation,and the interaction between Src and GlyR-α3 was enhanced in ipsilateral DRG com-pared with contralateral side.Inhibition of Src kinase activity helped to reduce tyrosine phosphorylation of GlyR-α3 from DRG and alleviate postoperative pain symptoms.Conclusion Src kinase is involved in the development of postoperative pain by enhancing tyro-sine phosphorylation of GlyR-α3.
