Ginkgo biloba extract activates Nrf2/ARE pathway to improve vascular endothelial dysfunction induced by chronic intermittent hypoxia in rats
- VernacularTitle:银杏叶提取物激活Nrf2/ARE通路改善慢性间歇性低氧诱导大鼠血管内皮功能障碍
- Author:
Sheng-Yong SI
1
;
Hong-Man LI
;
Si-Si MIAO
;
Xiao HAN
;
Zhi-Jing LI
;
Chao-Jun WEI
;
Da-Nan LIU
Author Information
- Keywords: Ginkgo biloba extract; Nrf2/ARE path-way; chronic intermittent hypoxia; vascular endothelial dysfunction; hypertension; oxidative stress
- From: Chinese Pharmacological Bulletin 2024;40(10):1837-1844
- CountryChina
- Language:Chinese
- Abstract: Aim To investigate the effects of Ginkgo biloba extract(GBE)on vascular endothelial dysfunc-tion induced by chronic intermittent hypoxia(CIH)in rats and its related mechanisms.Methods The CIH rat model was established,and 50 and 100 mg·kg-1 GBE was administered by intragastric administration.The systolic blood pressure(SBP)of the tail artery was detected in each group.HE staining was used to detect the morphology of aorta tissue.DAF-FM DA staining and nitric reductase assay were used to detect NO levels.ELISA was used to detect serum ET-1,TNF-α and IL-6 levels.DHE staining was used to de-tect reactive oxygen species(ROS)levels of aortic tis-sue.Kits were used to detect the serum levels of MDA,SOD and GSH-Px.Western blot was used to detect the levels of VCAM-1,ICAM-1,nucleus Nrf2,HO-1 and NQO1 of aortic tissue.Results GBE sig-nificantly decreased the levels of SBP,ET-1,ROS,MDA,VCAM-1,ICAM-1,TNF-α and IL-6,and sig-nificantly increased the levels of NO,SOD,GSH-Px,nuclear Nrf2,HO-1 and NQO1 in CIH rats.GBE sig-nificantly improved the histomorphology of aorta in CIH rats.Conclusions GBE can improve vascular endo-thelial dysfunction and reduce blood pressure in CIH model rats.The mechanism may be related to the acti-vation of Nrf2/ARE pathway and the inhibition of oxi-dative stress and inflammation by GBE.
