Study on mechanism of Tibetan medicine Rhodiola crenulata in treatment of cerebral microcirculatory disorders based on network pharmacology and experimental validation in rats
10.12360/CPB202309010
- VernacularTitle:基于网络药理学和大鼠体内验证的藏药红景天改善脑微循环障碍作用机制研究
- Author:
Si-Qing MA
1
;
Yu-Jing SHI
;
Yuan-Bai LI
;
Yang YANG
;
Meng LI
;
Yu DU
;
Yi-Hao LI
;
Fang-Zhou LIU
Author Information
1. 青海省人民医院,青海西宁 810007
- Keywords:
cerebral microcirculation disorder;
Tibet-an medicine;
Rhodiola crenulate;
network pharmacolo-gy;
reverse pharmacophore matching;
molecular doc-king;
RT-qPCR;
western blot;
mechanism
- From:
Chinese Pharmacological Bulletin
2024;40(9):1781-1791
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the core target,key com-ponents and mechanism of Tibetan medicine Rhodiola crenulata in improving cerebral microcirculation based on literature research,network pharmacology,molecu-lar docking and experimental verification.Methods The chemical components of Rhodiola were collected through literature and database,and the potential tar-gets of Rhodiola crenulata were predicted by reverse pharmacophore matching.The related targets of cere-bral microcirculation disorder were obtained and targets were mapping with Rhodiola crenulata.PPI network was constructed and the core targets were screened.The regulatory network of"herb-component-target-dis-ease"was constructed and key components were screened.GO and KEGG enrichment analysis were conducted,and a"Core target-Pathway-Biological Process"network was constructed.Finally,molecular docking validation was carried out,and RT-qPCR and Western blot were used for animal experiments to fur-ther confirm the results of network pharmacology analy-sis.Results A total of 76 active components of Rhodiola crenulata were obtained and corresponding to 285 targets.Altogether 1074 related targets related to cerebral microcirculation disorder were obtained.A-mong them,there were 97 common targets and the main core targets were 6.The key components were 6.The results of molecular docking showed that the bind-ing activity of three key components to the core target was greater than that of the core target protein and its original ligand.The result of RT-qPCR and Western blot demonstrated that Tibetan medicine Rhodiola cre-nulata could significantly reduce the expression of core target CASP3 and AKT1(P＜0.01).Conclusions Tibetan medicine Rhodiola crenulata can improve the cerebral microcirculation disorder through multi compo-nents,multi targets and multi pathways.This study provides an experimental basis for clinical application of Tibetan medicine Rhodiola crenulata to treat cerebral microcirculation disorder.
