Vanillin down-regulates cGAS/STING signaling pathway to improve liver tissue injury in rats with intrahepatic cholestasis
10.12360/CPB202401031
- VernacularTitle:香兰素下调cGAS/STING信号通路改善肝内胆汁淤积大鼠肝组织损伤
- Author:
Ning JIANG
1
;
Lan-Xiang PU
;
Feng HUANG
;
Yan WANG
;
Xin PEI
;
Jun-Ya SONG
;
En-Sheng ZHANG
Author Information
1. 青岛大学附属山东省妇幼保健院儿科
- Keywords:
vanillin;
cyclic guanylate adenylate syn-thetase;
stimulator of interferon gene;
intrahepatic cho-lestasis;
liver injury;
liver fibrosis
- From:
Chinese Pharmacological Bulletin
2024;40(9):1695-1700
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of vanillin on the regulation of cyclic guanylate adenylate synthetase(cGAS)/stimulator of interferon gene(STING)signa-ling pathway on hepatic tissue injury in rats with intra-hepatic cholestasis(IC).Methods SD rats were randomly divided into normal group,IC group,vanillin group,cGAS overexpression group,and vanillin+cGAS overexpression group,with continuous adminis-tration for seven days.The body weight,liver weight and liver to body weight ratio of rats were measured.Liver function(ALT,AST,ALP,LDH),IC(TBIL,TBA)and liver fibrosis(HA,LN,PC Ⅲ)index were determined by ELISA.Liver pathology and fibrosis were observed using HE and Masson staining,and col-lagen volume fraction was calculated.The expression of cGAS/STING pathway related proteins in liver tissue was detected by Western blot.Results Vanillin could improve liver pathology and fibrosis,increase body weight,and decrease liver weight,ALT,AST,ALP,LDH,TBIL,TBA,HA,LN,PC Ⅲ,collagen volume fraction,cGAS,STING protein in IC rats(P＜0.05).Overexpression of cGAS could reverse the effects of vanillin on the above indicators in IC rats(P＜0.05).Conclusions Vanillin may improve liver function,IC,liver fibrosis,and liver tissue damage in IC rats by downregulating the cGAS/STING signaling pathway.
