Inhibition effect of kudinoside D on lipid deposition in hepatocytes and its mechanism
- VernacularTitle:苦丁冬青苷D抑制肝细胞脂质沉积作用及机制
- Author:
Cai-Cai XUE
1
;
Yan-Xiang LI
;
Xiu-Mei QIAO
;
Jin-Yong PENG
;
Jin-Hong WANG
Author Information
- Keywords: kudinoside D; AML-12 cells; palmitic acid; lipid deposition; oxidative stress; non-alcoholic fatty liver disease
- From: Chinese Pharmacological Bulletin 2024;40(9):1688-1694
- CountryChina
- Language:Chinese
- Abstract: Aim To investigate the effect of kudinoside D(KD-D)on palmitic acid(PA)-induced lipid depo-sition in hepatocytes.Methods Mouse hepatocytes AML-12 were cultured and randomly divided into the Control group,PA group,PA+KD-D 20 μmol·L-1 group,PA+KD-D 40 μmol·L-1 group and PA+KD-D 80 μmol·L-1 group.AML-12 cells in PA and KD-D groups were treated with PA(0.4 mmol·L-1)for 24 h.AML-12 cells in KD-D groups were incubated with KD-D for 1 h before stimulation with PA.MTT as-say was used to detect cell survival rate,oil red O stai-ning and transmission electron microscopy were used to detect lipid deposition in cells,DCFH-DA fluorescence probe was used to detect intracellular reactive oxygen species(ROS)and MitoSOX mitochondrial superoxide red fluorescence probe was used to detect mitochondrial superoxide content in cells.Results KD-D at differ-ent concentrations improved PA-induced changes in cell morphology significantly.Compared with the Con-trol group,cells in PA group showed a significant in-crease in intracellular lipid droplets.Compared with PA group,the red lipid droplets in KD-D groups de-creased.The results of transmission electron microsco-py demonstrated that KD-D reduced PA-induced hepat-ic steatosis and improved ultrastructure.In addition,KD-D significantly decreased PA-induced cellular ROS level(P＜0.01)and reduced mitochondrial superox-ide content(P＜0.01).Conclusion KD-D inhibits PA-induced lipid deposition by regulating the cellular oxidative stress levels in AML-12 cells.
