Exploring mechanism of action of Xihuang pills against hyperplasia of mammary glands based on quantitative proteomics
10.12360/CPB202402049
- VernacularTitle:基于定量蛋白质组学探讨西黄丸抗乳腺增生的作用机制
- Author:
Rui TAO
1
;
Jing-Rui WANG
;
Jun-Liang WANG
;
Xue-Li MA
;
Juan-Xia SUN
;
Guang-Miao GAO
;
Qi-Rui FAN
;
Tao HAN
Author Information
1. 甘肃中医药大学中药学教研室
- Keywords:
Xihuang pills;
proteomics;
hyperplasia of mammary glands;
BIN1 protein;
ACLY protein;
AL-DOC protein
- From:
Chinese Pharmacological Bulletin
2024;40(9):1641-1648
- CountryChina
- Language:Chinese
-
Abstract:
Aim To clarify the differential proteins of mammary tissues in Xihuang pills(XHP)against hy-perplasia of mammary glands(HMG)based on quanti-tative proteomics technology and validate them,and to explore the mechanism of action.Methods SD rats were randomly divided into blank group,model group and XHP group,with 10 rats in each group.Except for the blank group,estrogen and progesterone were injec-ted intramuscularly to establish a rat model of mamma-ry hyperplasia for 30 d.After XHP was administered for 14 d,the rats in each group were observed to have morphological changes in the apparent morphology of the mammary tissues,and pathological changes in the mammary tissues were stained with hematoxylin-eosin staining(HE),and the differentially expressed pro-teins(DEPs)in the groups were screened by quantita-tive proteomics technology and subjected to bioinforma-tics analysis,and Western blot to verify the key DEPs.Results Compared with the model group,the appar-ent pathological morphology of the XHP group was sig-nificantly improved,the diameter of the nipple height of the rats was significantly reduced(P＜0.01),and the degree of histopathology was significantly allevia-ted.Quantitative proteomics identified 4,299 DEPs in mammary tissue,and bioinformatics analysis of 14 DEPs with consistent changes between the XHP group and the blank group relative to the model group re-vealed that they were related to the regulation of mus-cular systemic processes,regulation of muscle contrac-tion,DNA replication,and pre-initiation of DNA repli-cation.Western blot results showed that,compared with the model group,rat mammary tissue of the XHP group showed significantly lower levels of ACLY and ALDOC protein expression levels were significantly re-duced and BIN1 protein expression levels were signifi-cantly increased(P＜0.01).Conclusions XHP may exert its anti-mammary hyperplasia effect through the regulation of BIN1,ACLY and ALDOC protein lev-els,the regulation of DNA replication,the regulation of pre-initiation of DNA replication and muscular sys-temic processes,and the regulation of muscle contrac-tion.
