The protective effect of Jujing formula on tretina of mice with dry age-related macular degeneration
- VernacularTitle:菊睛方对碘酸钠诱导的干性年龄相关性黄斑变性小鼠视网膜的保护作用及物质基础研究
- Author:
Cheng-Cheng QI
1
;
Ruo-Ying FAN
;
Xue-Sen WANG
;
Shu-Lan SU
;
Yue ZHU
;
Sheng GUO
;
Hong-Jie KANG
;
Xue-Yi ZHOU
;
Jin-Ao DUAN
Author Information
- Keywords: Jujing formula; dry age-related macular degeneration; retinal injury; anti-oxidation; inflamma-tory factor; apoptosis
- From: Chinese Pharmacological Bulletin 2024;40(7):1358-1367
- CountryChina
- Language:Chinese
- Abstract: Aim To investigate the protective effect of Jujing formula on retina of mice with dry age-related macular degeneration(AMD).Methods The mouse model of dry AMD was induced by intraperitoneal in-jection of sodium iodate,and the prognosis was given to the Jujing formula.Retinal thickness was detected by optical coherence tomography(OCT),the retinal morphological changes were observed by hematoxylin-eosin(HE)staining,and the apoptosis of retinal cells was detected by in situ terminal transferase labeling(TUNEL)staining.Combination of tumor necrosis fac-tor-α(TNF-α),interleukin-6(IL-6)and interleukin-1β(IL-1 β)in eyeballs and serum,superoxide dis-mutase(SOD),glutathione(GSH)and malondialde-hyde(MDA)were evaluated to assess the protective effects of Jujing formula on retinal injury in mice with dry AMD.Results The results of OCT,HE and TUNEL staining showed that Jujing formula significant-ly improved the retinal injury induced by sodium iodate in mice with dry AMD,increased the retinal thickness(P＜0.05),reduced the apoptosis of retinal cells(P＜0.01),and increased the levels of GSH,IL-6 and SOD activity in eyeballs and serum(P＜0.01).The levels of TNF-α,IL-6,IL-1β and MDA were reduced(P＜0.01).Conclusions Jujing formula has certain therapeutic effects on retinal injury in dry AMD,which may be related to inhibiting inflammatory response and enhancing antioxidant capacity.
