Effects of GanoExtra combined with CTX on lung metastasis and immune function in mice
10.12360/CPB202412088
- VernacularTitle:灵芝康爱芝宝增强环磷酰胺抑制小鼠乳腺癌肺转移的增效减毒作用
- Author:
Shu LIAN
1
;
Ting-Jian WU
;
Jie CHEN
;
Chun-Lian ZHONG
;
Yu-Sheng LU
;
Ye LI
;
Chang-Hui WU
;
Kun ZHANG
;
Li JIA
;
Xiao-Dong XIE
Author Information
1. 闽江学院材料与化学工程学院
- Keywords:
Ganoderma lucidum Kangai Zhibao(Ga-noExtra);
cyclophosphamide(CTX);
efficacy enhan-cing and toxicity;
CD8+T cells;
tumor metastasis;
im-mune function
- From:
Chinese Pharmacological Bulletin
2024;40(7):1335-1342
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the enhanced efficacy and reduced toxicity of GanoExtra in combination with cyclophosphamide(CTX)on inhibiting lung metastasis and immune function in mice.Methods The CCK-8 method was used to verify the cytotoxic effects of Gano-Extra on MCF-7 and 4T1 tumor cells.In vivo experi-ment,a mouse model of lung metastasis of breast canc-er was established by injecting 4T1 tumor cells into the tail vein,which was divided into four groups including 4T1 model group,CTX group,GanoExtra group and GanoExtra+CTX group.The control group was set.After 21 days,the mice were euthanized under anes-thesia,and the body weight of the mice was recorded.Average lung index and spleen index were calculated.The mouse spleen lymphocyte transformation experi-ment was used to determine the activity of spleen cells.The NK cell activity assay was used to determine the activity of NK cells.Blood cells were determined in mouse blood samples.Flow cytometry was used to de-termine the levels of CD4+and CD8+T cells in blood samples.ELISA was used to measure the levels of TNF-α and IL-6 in serum.HE staining was used to ob-serve the pathological morphological changes in tumors and various tissues;and CFSE staining was used to de-termine the proliferative effect of GanoExtra on CD8+cells.Results In vitro GanoExtra at 50 mg·L-1 sig-nificantly inhibited the activity of MCF-7 and 4T1 tumor cells.In the breast cancer pulmonary metastasis model,compared with the model group,the spleen in-dex and blood WBCs content were significantly re-duced,while the activity of NK cells,spleen cells,and the proportion of RBCs,CD 3+and CD 8+T cells in the blood were significantly increased.At the end of the treatment,compared with the CTX group,the number of lung metastases and lung index of the Gano-Extra+CTX group were significantly reduced,and the levels of HGB,CD8+cells,IL-6,and TNF-α in the blood of mice were significantly increased.GanoExtra at 10 mg·L-1 significantly promoted the proliferation of CD8+T cells in vitro.Conclusions GanoExtra can enhance the inhibitory effect of CTX on tumor metasta-sis,alleviate adverse reactions such as splenomegaly and pulmonary enlargement caused by CTX,and have a health-promoting function of promoting the prolifera-tion of CD8+T cells to enhance the immune efficacy of the body.
