Targeting B7-H3 inhibited growth,migration,and angiogenesis of human umbilical vein endothelial cells
10.12360/CPB202311106
- VernacularTitle:靶向B7-H3抑制人脐静脉血管内皮细胞生长、迁移和血管形成
- Author:
Yu LIU
1
;
Nan-Nan WANG
;
Ke NIU
;
Hui-Juan LING
;
Ya-Yu ZHU
;
Wei HU
;
Li-Wen CHEN
Author Information
1. 安徽医科大学第二附属医院检验科
- Keywords:
co-signaling molecules;
angiogenesis;
targeted drugs;
small interfering RNA;
human umbili-cal vein endothelial cells;
cell migration
- From:
Chinese Pharmacological Bulletin
2024;40(7):1311-1316
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the effect of targeted inhi-bition of co-signaling molecule B7-H3 on the growth,migration,and angiogenesis ability of human umbilical vein endothelial cells(HUVECs).Methods Small interference RNA was used to knock down HUVECs B7-H3 molecules.CCK-8 test was used to detect cell proliferation at 24 h,48 h and 72 h.Transwell test was then used to detect 24 h cell migration,and three-dimensional cell culture was used to observe cell angio-genesis.Results Compared with the negative control group(siRNA-Control),siRNA-720,siRNA-1707 and siRNA-1690 had different inhibitory effects on the expression of B7-H3.B7-H3 inhibition of siRNA-1690 was significantly higher than that of siRNA-720 and siRNA-1707,and siRNA-1690 sequence was chosen for follow-up experiment.The results of CCK-8 cell vi-ability assay showed that the proliferation ability of HU-VECs decreased by 24％,22％(P＞0.05,compared with 24 h)and 15％(P＜0.05,compared with 48 h)respectively at 24 h,48 h and 72 h after B7-H3 knockout.The migration ability of B7-H3 for 24 h was significantly lower than that of siRNA-Control group(P＜0.01).The results of three-dimensional cell cul-ture showed that the angiogenic ability of HUVECs de-creased significantly after si-B7-H3 knockdown of B7-H3 gene(P＜0.01).Conclusion Targeting B7-H3 inhibits the growth,migration,and angiogenesis of hu-man umbilical vein endothelial cells.
