Effect of Bushen Tongfu decoction on neuroinflammation in AD mice based on intestinal flora and metabolomics
- VernacularTitle:基于肠道菌群与代谢组学探讨补肾通腑方对AD小鼠神经炎症的影响
- Author:
Jie ZHANG
1
;
Xu WANG
;
Xiao-Yu SONG
;
Si-Min LI
;
Jian-Ping DUAN
;
Yun-Min XU
;
Min ZHAO
Author Information
- Keywords: Alzheimer's disease; Bushen Tongfu de-coction; learning and memory ability; intestinal flora; non-targeted metabolomics; neuroinflammation
- From: Chinese Pharmacological Bulletin 2024;40(7):1258-1266
- CountryChina
- Language:Chinese
- Abstract: Aim To explore the mechanism of Bushen Tongfu decoction(BSTF)improving the learning and memory ability of APP/PS1 mice.Methods The APP/PS1 mice were administered by BSTF for eight weeks.The spatial learning and memory ability of all mice were detected by Morris water maze.The changes in the intestinal microbiota and gut metabolites of mice were detected by 16S rDNA and LC-MS/MS technolo-gy.Results Compared with the model group,the es-cape latency and swimming path in BSTF-H,BSTF-M and BSTF-L groups were shortened and the times of target crossings after removing the platform increased significantly(P＜0.01).The 16S rDNA results showed that BSTF could increase the abundance of beneficial bacteria of Firmicutes and reduce the abun-dance of harmful bacteria of Bacteroidota in APP/PS1 mice.A total of 25 differential metabolites were identi-fied by LC-MS/MS,including sphingolipid metabo-lism,lysine metabolism and tyrosine metabolism.KEGG enrichment analysis showed that the therapeutic effect of BSTF was mainly through inflammation-related signaling pathways such as NF-κB pathways and Apelin pathways.Conclusions The mechanism of BSTF im-proving the learning and memory ability of APP/PS1 mice may be related to the improvement of intestinal flora composition,the effect of sphingolipid metabo-lism,lysine metabolism and tyrosine metabolism and the regulation of inflammation-related signaling path-ways such as NF-κB and Apelin pathways.
