Preliminary study on delaying aging induced thymus degeneration in SAMP6 mice with Bazi Bushen capsule
10.12360/CPB202309016
- VernacularTitle:八子补肾胶囊延缓SAMP6小鼠增龄性胸腺退化的初探
- Author:
Zhao-Dong LI
1
;
Yin-Xiao CHEN
;
Bo-Yang GONG
;
Zhe XU
;
Zhi-Xian YU
;
Yue-Xuan SHI
;
Yan-Fei PENG
;
Yu-Hong BIAN
;
Yun-Long HOU
;
Xiang-Ling WANG
;
Shu-Wu ZHAO
Author Information
1. 天津中医药大学中西医结合学院
- Keywords:
Bazi Bushen capsules;
thymus involu-tion;
immunosenescence;
thymic epithelial cell;
IL-7;
SAMP6 mice
- From:
Chinese Pharmacological Bulletin
2024;40(6):1186-1192
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the improvement effect of Bazi Bushen capsule on thymic degeneration in SAMP6 mice and the possible mechanism.Methods Twenty 12 week old male SAMP6 mice were randomly divided into the model group(SAMP6)and the Bazi Busheng capsule treatment group(SAMP6+BZBS).Ten SAMR1 mice were assigned to a homologous control group(SAMR1).The SAMP6+BZBS group was oral-ly administered Bazi Bushen capsule suspension(2.8 g·kg-1)daily,while the other two groups were orally administered an equal amount of distilled water.After nine weeks of administration,the morphology of the thymus in each group was observed and the thymus in-dex was calculated;HE staining was used to observe the structural changes of thymus tissue;SA-β-gal stai-ning was used to detect thymic aging;flow cytometry was used to detect the proportion of thymic CD3+T cells in each group;Western blot was used to detect the levels of p16,Bax,Bcl-2,and cleaved caspase-3 proteins in thymus;immunofluorescence was applied to detect the proportion of cortical thymic epithelial cells in each group;ELISA was employed to detect IL-7 lev-els in thymus.Results Compared with the SAMP6 group,the thymic index of the SAMP6+BZBS group significantly increased(P＜0.05);the disordered thy-mic structure was significantly improved;the positive proportion of SA-β-gal staining significantly decreased(P＜0.01);the proportion of CD3+T cells apparently increased(P＜0.05);the level of p16 protein signifi-cantly decreased(P＜0.05);the level of Bcl-2 pro-tein significantly increased(P＜0.05),while the lev-el of cleaved caspase-3 protein markedly decreased(P＜0.05);the proportion of cortical thymic epithelial cells evidently increased;the level of IL-7 significantly increased(P＜0.01).Conclusions Bazi Bushen capsule can delay thymic degeneration,inhibit cell ap-optosis in thymus and promote thymic cell development in SAMP6 mice,which may be related to increasing the proportion of cortical thymic epithelial cells and promoting IL-7 secretion.
