Study on improvement effect of Danggui Shaoyao San on edema in rats with nephrotic syndrome and its mechanism
- VernacularTitle:当归芍药散对肾病综合征大鼠水肿的改善作用及机制
- Author:
Xiao-Wen MA
1
;
Sheng-Nan FAN
;
Zai-Ping XU
;
Qing-Zhen XIANG
;
Zi-Hua XUAN
;
Yun-Lai WANG
;
Fan XU
Author Information
- Keywords: nephrotic syndrome; edema; aquaporin 2; arginine vasopressin; angiotensin Ⅱ; Danggui Shaoyao San
- From: Chinese Pharmacological Bulletin 2024;40(6):1172-1178
- CountryChina
- Language:Chinese
- Abstract: Aim To explore the effect of Danggui Shaoyao San on edema in rats with nephrotic syndrome and the underlying mechanism.Methods Rats were randomly divided into control group,model group,Danggui Shaoyao San group(17.2 g·kg-1·d-1),losartan group(30 mg·kg-1·d-1)and tolvaptan group(3 mg·kg-1·d-1).The rat model of nephrot-ic syndrome was established by tail vein injection of adriamycin.After four weeks of treatment,the levels of renal function and 24 h urinary protein were detected.The distribution of aquaporin 2(AQP2)and pS256-AQP2 in renal tissue was detected by immunohisto-chemistry.The levels of plasma arginine vasopressin(AVP)and angiotensin Ⅱ(Ang Ⅱ)were measured by radioimmunoassay.The expressions of renal AQP2,pS256-AQP2,angiotensin type 1 receptor(AT1R),arginine vasopressin receptor 2(V2R)protein and mRNA were measured by Western blot and RT-PCR,respectively.Results The three drugs could improve renal function,reduce proteinuria,decrease plasma AVP and Ang Ⅱ levels,and down-regulate AQP2 and pS256-AQP2 protein and mRNA expression in model rats.Danggui Shaoyao San and tolvaptan were more ef-fective than losartan in reducing plasma AVP levels.Conclusions Danggui Shaoyao San may regulate the expression of AQP2 by reducing the levels of AVP and Ang Ⅱ,and improve the edema of nephrotic syndrome rats.
