Carthamus tinctorius L.extract ameliorates alcoholic liver disease by modulating PI3K/Akt/FoxO signaling pathway
- VernacularTitle:红花提取物通过调节PI3K/Akt/FoxO信号通路改善酒精性肝病
- Author:
Wen-Xuan WANG
1
;
Xiang-Lei FU
;
Man QI
;
Fu-Rong FAN
;
Fu-Rong ZHU
;
Yuan-Chuang WANG
;
Kai-Yue ZHANG
;
Min LIU
;
Sheng-Hui CHU
Author Information
- Keywords: Carthamus tinctorius L.extract; lipid me-tabolism; alcohol-associated liver disease; oxidative stress; apoptosis; PI3K/Akt/FoxO signaling pathway
- From: Chinese Pharmacological Bulletin 2024;40(6):1137-1145
- CountryChina
- Language:Chinese
- Abstract: Aim To investigate the effects of Cartham-us tinctorius L.extract(CTLE)on oxidative stress,lipid metabolism,and apoptosis levels of mice with al-cohol-induced liver injury and its mechanism of action.Methods The mouse model of alcohol-associated liver disease was established by chronic alcohol feeding and acute alcohol gavage.Mice were randomly divided into four groups.During the modeling period,the state changes of mice were observed every day,and their weight was recorded.At the end of modeling,blood and liver tissues were collected from each group of mice.The blood of mice was analyzed biochemically,and HE staining and Oil Red O staining were used to evaluate further the degree of pathological damage in the liver of mice.Quantitative real-time PCR(qPCR)and Western blot were applied to detect the mRNA and protein expression levels of p-PI3K,PI3K,p-Akt,Akt,p-mTOR,mTOR,p-FoxO1,FoxO1,p-FoxO3a,FoxO3a,p-FoxO4,FoxO4,BCL and BAX factors.Results Compared to the model group,the CTLE administration group showed improved hepatic patho-logical injury and reduced lipid deposition.The bio-chemical indexes in serum and liver,such as ALT,AST,TG,TC,and MDA levels were reduced,while GSH and SOD levels increased.Regulating the PI3K/Akt/FoxO pathway resulted in increased production of SOD,which reduced damage and apoptosis caused by reactive oxygen species(ROS).Conclusions CTLE can exert anti-oxidative stress and anti-apoptotic effects through the PI3K/Akt/FoxO pathway and attenuates alcoholic liver injury in mice,providing new ideas for the treatment of alcoholic liver disease and the develop-ment of related drugs.
